Serena Williams and Venus Williams of the USA are seen during their match against Lucie Safarova and Barbora Strycova of Czech Republic in the 2016 Rio Olympics women's doubles first round match at the Olympic Tennis Center in Rio de Janeiro, Brazil, Aug 7, 2016. [Photo/Agencies]

RIO DE JANEIRO - Three-time Olympic women's doubles champions Serena and Venus Williams crashed out of the Rio Games tournament in the first round on Sunday as the American sisters' unbeaten run dating back to Sydney 2000 came to a surprising end.

The top seeds fell 6-3 6-4 to Czech pair Barbora Strycova and Lucie Safarova, ending any hopes of claiming a third successive Olympics doubles crown after they won gold in Beijing eight years ago and again at London 2012.

The sisters won their first doubles gold in Sydney but a knee injury prevented Serena from playing at Athens in 2004. They have also won 14 women's doubles Grand Slam titles, to go along with a combined tally of 29 major singles crowns.

After their loss, Serena was philosophical about the result and preferred to focus on the success of one of the greatest ever doubles pairings.

"I wouldn't stay it's devastating... we had a chance to compete for our country, we did the best that we can. We had a blast out there," Serena, 34, told reporters.

"We've had so many Olympic doubles golds, so many Grand Slam championships. It's been unbelievable."

Both the sisters have also won Olympic gold medals in singles play, with Venus triumphing in Sydney and then Serena becoming champion in London.

Earlier on Sunday, Serena crushed Australia's Daria Gavrilova 6-3 6-2 in her first round singles match, a day after Venus was eliminated by Belgium's Kirsten Flipkens.